Part:BBa_K4432141:Design

Pseudomonas aeruginosa PhzA2-G2 operon under the control of the T5 promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1052
  Illegal EcoRI site found at 3674
  Illegal PstI site found at 1195
  Illegal PstI site found at 4679
  Illegal PstI site found at 5228
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1052
  Illegal EcoRI site found at 3674
  Illegal PstI site found at 1195
  Illegal PstI site found at 4679
  Illegal PstI site found at 5228
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1052
  Illegal EcoRI site found at 3674
  Illegal BamHI site found at 395
  Illegal BamHI site found at 1607
  Illegal XhoI site found at 339
  Illegal XhoI site found at 498
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1052
  Illegal EcoRI site found at 3674
  Illegal PstI site found at 1195
  Illegal PstI site found at 4679
  Illegal PstI site found at 5228
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1052
  Illegal EcoRI site found at 3674
  Illegal PstI site found at 1195
  Illegal PstI site found at 4679
  Illegal PstI site found at 5228
  Illegal NgoMIV site found at 261
  Illegal NgoMIV site found at 1276
  Illegal NgoMIV site found at 1423
  Illegal NgoMIV site found at 1441
  Illegal NgoMIV site found at 1445
  Illegal NgoMIV site found at 2249
  Illegal NgoMIV site found at 2423
  Illegal AgeI site found at 5517
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5660
  Illegal BsaI.rc site found at 387
  Illegal BsaI.rc site found at 911
  Illegal BsaI.rc site found at 2359
  Illegal BsaI.rc site found at 4736
  Illegal BsaI.rc site found at 5142
  Illegal BsaI.rc site found at 5169
  Illegal SapI.rc site found at 4156
  

Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432015) + Pseudomonas aeruginosa PhzA2-G2 operon (BBa_K4432041)

Source

PCR amplification & Assembly